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AMB Express
Fig. 5 | AMB Express

Fig. 5

From: An improved integrative GFP-based vector for genetic engineering of Parageobacillus thermoglucosidasius facilitates the identification of a key sporulation regulator

Fig. 5

A The genomic composition of the spo0A region in P. thermoglucosidasius in the wildtype strain and following the deletion of spo0A. B Colony PCR of the spo0A region (primer set PNJ1245/PNJ1246) performed on second recombinants, as identified through lack of fluorescence. Note how some colonies have only looped out the pMM7, thus reverting to wildtype (matching wildtype control ‘WT’). The remaining colonies have looped out spo0A alongside pMM7, and the resulting bands are therefore significantly shorter. The band of the ∆spo0A colony selected for further verification and testing is marked with an arrow

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