Table 1 Bacterial strains and plasmids used in this study
Strain | Genotype or description | Source/Reference |
|---|---|---|
E. coli NEB® turbo | F’ proA + B + lacIq ∆lacZM15 / fhuA2 ∆(lac-proAB) glnV galK16 galE15  R(zgb-210::Tn10)TetS | Laboratory stock |
B. megaterium DSM 319 | Source of fpps gene | DSM |
B. subtilis 168 KO7-S | ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr ΔsigF | BGSC |
BsMN0 | B. subtilis KO7-S strain harboring the plasmid pHY_crtMN | This study |
BsMN1 | Contains one crtMN gene-copy integrated into the genome | Ferrando et al., 2023 |
BsMN2 | Contains two crtMN gene-copies integrated into the genome | Ferrando et al., 2023 |
BsMN3 | Contains three crtMN gene-copies integrated into the genome | Ferrando et al., 2023 |
BsMN4 | B. subtilis KO7-S strain harboring the plasmid pBS0E_crtMN | This study |
BsMN5 | BsMN3 strain with an fpps gene from B. megaterium replacing the sigX gene | This study |
BsMN6 | BsMN5 strain with a truncated copy of the yisP gene | This study |
Plasmid | Description | Source/Reference |
pHY_crtMN | Plasmid pHY300PLK containing the crtMN operon from Staphylococcus aureus | Yoshida et al. 2009 |
pBS0E | Plasmid containing the xylose-inducing promoter xylose-repressor system | Popp et al. 2017 |
pJOE8999 | PmanP-cas9, pUC, pE194ts, kanr | Altenbuchner 2016 |
pJOE8999_VG_MN | Plasmid used to replace the spoVG locus for the crtMN genes | Ferrando et al., 2023 |
pBS0E_crtMN | Plasmid containing crtMN genes under the control of xylose-inducing promoter | This study |
pJOE8999.sg_sigX_fpps | Plasmid used to replace the sigX locus for the fpps gene | This study |
pJOE8999.sg_ΔyisP | Plasmid used to delete the yisP gene | This study |