Fig. 2
From: Overexpression of miR-32 in Chinese hamster ovary cells increases production of Fc-fusion protein
VEGF-ELISA and SDS-PAGE results. a Indicates the result of VEGF-ELISA. The standard aflibercept and CHO-K1 supernatant have been used as positive and negative control respectively. Error bars represent standard deviation of technical replicate (n = 3). The stars denote the significance of differences (p < 0.05). b Indicates the reduced SDS-PAGE gel result of purified VEGF-trap produced by selected single clone. The samples were subjected to reducing (R) SDS-PAGE assay and the band of approximately 70 kDa was observed, as expected, L1 and L2 are different purified fractions, L4 standard aflibercept, L6, protein marker (10–250 kDa) L3 and L5 are empty