Fig. 5

Conservation of lanthipeptide leader sequence over diverse core sequences. A Multiple sequence alignment using MUSCLE of all the identified 68 precursor peptides from 42 BGCs. All leader sequences are from the NHLP family of lanthipeptide leaders. Conserved residue positions are highlighted. B Pairwise identity among 68 lanthipeptide leader sequences (lower half) and core sequences (upper half) showed high similarity among leader over core sequences (except lanthipeptide core sequences of the roseocin family having core conservation). C Cumulative frequency of pairwise sequence identity among the lanthipeptide leader and core sequences, respectively. 80% of lanthipeptide leader pairwise alignment showed > 39% identity. However, only 10% of lanthipeptide core pairwise alignment fulfilled the same criteria (mainly of the roseocin family)