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Table 1 Strain and plasmid used in study

From: Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Name

Characteristic

Ref.

Strain

  

 JM109

recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ (lac proAB)/F, [traD36, proAB + , lacqlacZ∆M15]

Takara

 BS168

Bacillus subtilis168

Lab stock

 BS01

Bacillus subtilis168 derivate, BS168∆bdhA, ∆acoA

This study

 BS02

BS01 derivate, expressing araE gene under the control of HpaII promoter via the plasmid pMA5

This study

 BS03

BS01 derivate, expressing araE,galKTE gene under the control of the HpaII promoter via the plasmid pMA5

This study

 BS04

BS01 derivate, BS01 ∆ptsG, ∆yyzE, ∆ypqE, expressing araE, galKTE gene under the control of HpaII promoter via the plasmid pMA5

This study

Plasmids

  

 pMA5

Amp, Kana, E.coli-Bacillus shuttle vector, HpaII promoter

Lab stock

 pMA5-araE

pMA5 harboring araE gene under the control of HpaII promoter

This study

 pMA5-araE-galKTE

pMA5 harboring araE, galKTE gene under the control of HpaII promoter

This study