Fig. 1
From: Cloning and expression of an anti-cancerous cytokine: human IL-29 gene in Chlamydomonas reinhardtii
Generation and confirmation of IL29 transformants. a The pSRSapI-IL29 plasmid contains IL29 under the control of the psaA exon-1 promoter/5′UTR and rbcL 3′UTR. This construct comprises psbH mutated in the TN72 strain of Chlamydomonas reinhardtii. b PCR confirmation of IL29 integration in pSRSapI expression vector through gene-specific primers. Transformants yield the 564 bp product