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Fig. 4 | AMB Express

Fig. 4

From: Immobilization of E. coli expressing γ-glutamyltranspeptidase on its surface for γ-glutamyl compound production

Fig. 4

Determining the reusability of the immobilized cells of SH2161. The transpeptidation (black circle) and the hydrolysis (white circle) activities were measured in the presence and absence of Gly-Gly at pH 8.73 for 20 min with 4 beads in the presence of 100 mM CaCl2 and 3% NaCl. After the reaction, the beads were washed and then incubated in 1.2 mL of 20 mM Tris-HCl (pH 7.4) with 100 mM CaCl2 at 37 °C for 22 h. This cycle was repeated 10 times. Values are expressed as the averages of three samples ± standard deviations

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