Fig. 2

Comparison of transpeptidation (gray bar) and hydrolysis (white bar) activities between the whole cells and the immobilized cells. Enzyme activity was assayed with 0.5 mM γ-GpNA, 50 mM Tris-HCl (pH 8.73), and 100 mM CaCl₂, with and without 60 mM Gly-Gly. The reaction was conducted at 37 °C for 20 min. Values are expressed as the averages of three samples ± standard deviations