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Fig. 1 | AMB Express

Fig. 1

From: Effects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes

Fig. 1

The central metabolic network for SA biosynthesis in A. succinogenes (a) (PEP, Phosphoenolpyruvate; Pyr, Pyruvate; LA, lactic acid; AA, acetic acid; FA, formic acid; ACALD, acetaldehyde; EtOH, Ethanol; OAA, oxaloacetate; Mal, malate; Fum, fumarate; SA, succinic acid; AcCoA, acetyl-CoA; AcP, acetyl-phosphate; pck, phosphoenolpyruvate carboxykinase; mdh, malate dehydrogenase; fum, fumarase; frd, fumarate reductase; pyk, pyruvate kinase; ldh, lactate dehydrogenase; pflB, pyruvate formate-lyase; pta, phosphoacetyltransferase); Schematic diagram of homologous recombination for ackA gene knockout (b); PCR analysis of the candidate A. succinogenes colonies (c) (M, 10 kb DNA ladder; WT, 3390 bp; ΔackA, 3114 bp)

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