Table 2 The kinetic parameters of wild-type and mutant dextranase AoDex
From: Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis
Dextranase | vmax (mmol·L−1·min−1) | Km (μmol·L−1) | kcat (s−1) | kcat/Km (μmol−1·L·s−1) × 103 |
|---|---|---|---|---|
Wild-type | 2.83 ± 0.05 | 50.9 ± 1.5 | 12.6 ± 0.2 | 247.8 ± 8.3 |
S357P | 4.83 ± 0.04 | 62.0 ± 1.3 | 11.1 ± 0.1 | 179.1 ± 4.1 |
S357F | 4.71 ± 0.15 | 115.6 ± 6.6 | 25.0 ± 0.8 | 217.0 ± 14.2 |
S357V | 4.26 ± 0.05 | 58.0 ± 1.5 | 20.3 ± 0.2 | 350.2 ± 9.7 |
S357I | 5.84 ± 0.02 | 121.5 ± 1.4 | 14.7 ± 0.1 | 121.0 ± 1.6 |
S357L | 4.89 ± 0.11 | 128.4 ± 5.2 | 12.8 ± 0.3 | 99.9 ± 4.6 |