Skip to main content
AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Development of fluorescence-labeled antibody for immune checkpoint inhibitor using engineered probiotics

Fig. 4

Purification of rGFP-PDL1scFv. a Chromatogram of protein elution. Protein adsorbed on a His-Trap column was eluted by adding imidazole. The eluted protein was collected as five fractions (F1–F5) based on A280. b The presence of rGFP-PDL1scFv in each fraction was analyzed by WB using an anti-His-tag antibody. c Results of SDS-PAGE (CBB staining) analysis. The black arrow indicates the band corresponding to the size of rGFP-PDL1scFv (59.0 kDa)

Back to article page