Fig. 3From: Development of fluorescence-labeled antibody for immune checkpoint inhibitor using engineered probiotics Immunoreactivity assay of NZ-GFP-PDL1scFv against PD-L1. A binding assay using an anti-His tag antibody was performed to verify the immunoreactivity of rGFP-PDL1scFv. Serially diluted cellular extracts of each gmLAB were added to wells immobilized (orange and black line) or not immobilized (gray line) with PD-L1 protein. The bound scFv was detected using an anti-His tag antibody, and the absorbance at 450 nm was measured. The data are representative of two independent experiments. Orange line: PD-L1+, NZ-GFP-PDL1scFv, Gray line: PD-L1-, NZ-GFP-PDL1scFv, Black line: PD-L1+, NZ-VCBack to article page