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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Development of fluorescence-labeled antibody for immune checkpoint inhibitor using engineered probiotics

Fig. 2

Gene expression and fluorescence analysis. NZ-VC, NZ-GFP, and NZ-GFP-PDL1scFv were cultured with/without nisin, and gene expression and fluorescence were analyzed. a The cell extracts were subjected to WB using an anti-His  tag antibody to detect the expression of rGFP-PDL1scFv by the gmLAB. The black arrow indicates the band corresponding to rGFP-PDL1scFv (59.0 kDa). The gray arrow indicates the band corresponding to rGFP (31.0 kDa). M: molecular mass marker (kDa). b Fluorescence analyses of gmLAB. Each gmLAB was observed by confocal laser scanning microscopy using an oil immersion objective lens (×60) under visible and fluorescent light, and merged images were generated. −/+: absence/presence of nisin stimulation

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