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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Development of fluorescence-labeled antibody for immune checkpoint inhibitor using engineered probiotics

Fig. 1

Scheme of plasmid construction. a The lactococcal gene expression vector, pNZ8148#2:CYT, derived from commercially available pNZ8148. b The green fluorescent protein (GFP) gene was integrated into pNZ8148#2:CYTo construct the lactococcal GFP expression vector, pNZ8148#2:CYT-GFP. c Predicted 3D model of PDL1scFv designed using SWISS-MODEL. The locations of the VH chain, VL chain, and peptide linker (EGKSSGSGSESKS) are shown. VH chain; variable region of the heavy chain, VL chain; variable region of the light chain. d The PDL1scFv gene was integrated into pNZ8148#2:CYT-GFP by restriction digestion using BamHI and HindIII to construct GFP-conjugated PDL1scFv expression vector, pNZ8148#2:CYT-GFP-PDL1scFv. PnisA: nisin-inducible promoter, His-tag six-histidine tag, MCS multiple cloning site, T terminator, repC and repA origins of replication, Cat chloramphenicol acetyltransferase, GFP green fluorescent protein

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