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Table 3 Kinetic parameters of GdhB for reductive amination and oxidative deamination reaction

From: Transcriptomic and enzymatic analysis reveals the roles of glutamate dehydrogenase in Corynebacterium glutamicum

Reaction

Substrate

Km \((\text{ mmol/L})\)

Vmax \((\text{ IU/mg})\)

Kcat \(({\text{mi}}{\text{n}}^{-1})\)

Kcat/Km \(({\text{min}}^{-1}\cdot \mathrm{L}\cdot {\text{mmol}}^{-1})\)

Reductive amination

NADPH

0.0382

1.539

26.61

696.779

α-KG

4.109

0.809

9.77

2.378

Oxidative deamination

NADP

0.412

1.341

6.42

15.581

glutamate

47.378

0.544

2.77

0.0586