Transcriptomic and enzymatic analysis reveals the roles of glutamate dehydrogenase in Corynebacterium glutamicum

AMB Express

Table 2 Kinetic parameters of GdhA for reductive amination and oxidative deamination reaction

Reaction	Substrate	K_m \((\text{ mmol/L})\)	V_max \((\text{ IU/mg})\)	K_cat \(({\text{mi}}{\text{n}}^{-1})\)	K_cat/K_m (min^− 1 L mmol^− 1)
Reductive amination	NADPH	0.166	1.539	15.39	92.655
Reductive amination	∝-KG	2.534	0.809	8.09	3.193
Oxidative deamination	NADP	0.505	1.341	13.41	26.554
Oxidative deamination	glutamate	174.856	0.544	5.44	0.0311