Table 1 Strains and plasmids used in this study
Strains and plasmids | Characteristic(s) | Sources |
|---|---|---|
Strain F1 | Wild type C.glutamicum F343 | Zheng et al. 2012 |
Strain F2 | C.glutamicum F343 ∆gdhA | Ge et al. 2021 |
Strain F3 | C.glutamicum F343 ∆gdhB | This study |
Strain F4 | C.glutamicum F343 ∆gltB | This study |
Strain F5 | C.glutamicum F343 ∆gdhA∆gdhB | This study |
Strain F6 | C.glutamicum F343 ∆gdhA∆gdhB∆gltB | This study |
pK18mobsacB | Mobilizable E.coli cloning vector, Kmr | Kvitko et al. 2011 |
pK18mobsacB-∆gdhB | Integrative transformation vector for deletion of the gdhB gene | This study |
pK18mobsacB-∆gltB | Integrative transformation vector for deletion of the gltB gene | This study |
E.coli DH5α | Wild-type strain; subcloning host | Laboratory stock |
E.coli TSBL21 (DE3) pLysS | λDE3 lysogenic bacteria, with T7 RNA polymerase and genes expressing T7 lysogenic bacteria | Laboratory stock |
E.coli BL21 (DE3) | Lysogenic λDE3 bacteria with T7 RNA polymerase | Laboratory stock |
E.coli TS setta (DE3) | With six rare codon AUA, AGG, AGA, CUA, CCC, and GGA corresponding TRNA | Laboratory stock |
pET28a( +) | E.coli expressing vector, Kmr | Laboratory stock |
pET28a( +)-gdhA | pET28a( +) harboring the gdhA gene | This study |
pET28a( +)-gdhB | pET28a( +) harboring the gdhB gene | This study |