Table 3 Aqueous two-phase system (ATPS) purification of Fusant-06 l-ASNase
From: Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase
Purification step | PEG Mol. wt | Total protein (mg) | Total activity (U) | Specific activity (Umg−1) | Yield (%) | Fold purification |
|---|---|---|---|---|---|---|
Crude extract | – | 1926.400 | 132007.00 | 68.525 | 100.00 | 1.00 |
ATPS | 6000 | 24.601 | 117935.05 | 4793.91 | 89.34 | 69.96 |
Sephadex G-100 | – | 8.036 | 80511.07 | 10019.04 | 60.99 | 146.21 |