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Table 2 Cost analysis of fermentation modes towards l-ASNase production by Aspergillus protoplast Fusant-06 and wild strains in a 3.5 L working volume bioreactor

From: Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase

Cost parameters

l-asparaginase producing strains under differing fermentation modes

Protoplast fusant

Aspergillus candidus

Aspergillus sydowii

Batch

Fed-batch

Continuous

Batch

Fed-batch

Continuous

Batch

Fed-batch

Continuous

Chemicals ($)

14.8

24.5

16.8

14.8

24.5

16.8

14.8

24.5

16.8

Consumables ($)

750

1150

1025

750

1150

1025

750

1150

1025

Labour ($50 h−1)

4900

10000

15000

5000

10000

15000

5000

10000

15000

Sum ($)

5664.8

11174.5

16041.8

5764.8

11174.5

16041.8

5764.8

11174.5

16041.8

DCW per vessel (g)

3066.49

8122.59

6050.065

2475.0

5294.34

4228.25

2599.24

4677.42

3938.45

Production cost per DCW ($g−1)

1.85

1.38

2.65

2.33

2.11

3.79

2.22

2.39

4.07

  1. DCW Dry cell weight; chemicals comprised media components employed to formulate production media, feeding nutrients and dilution nutrients; consumables comprised; labour cost comprised the cost of medium preparation, sterilizing, operating, cleaning, washing of bioreactors, and analysis of products (three employees for continuous mode, 2 for fed-batch and 1 for batch @50 $h−1 multiplied by 100 h; DCW per 5 L vessel with 3.5 L working volume; Total fermentation time = 100 h, from preparation through running to clean up