Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase

AMB Express

Table 1 Comparative kineto-technical analysis of l-ASNase production by protoplast Fusant-06 under submerged batch, fed-batch and continuous fermentation modes

FM	Biomass formation kinetic parameters by logistic model				Performance statistics of model
FM	X₀	X_max	µ_max	T_d	Adj. r²	RMSE	MAE	p-value
Batch	0.034 ± 0.000	876.14 ± 24.35	0.26 ± 0.01	2.67 ± 0.07	0.9987	14.59	11.06	< 0.0001
Fed-batch	0.000 ± 0.00	2061.84 ± 118.06	0.85 ± 0.01	0.815 ± 0.001	0.9995	20.53	10.53	< 0.0001
Con mode	0.01 ± 0.000	1688.72 ± 97.46	0.647 ± 0.01	1.071 ± 0.168	0.9992	20.25	8.34	< 0.0001

FM	l-ASNase formation model parameters by modified Gompertz model			Performance statistics of model
FM	P_max	rp_max	t_L	Adj. r²	RMSE	MAE	p-value
Batch	94048.10 ± 3225.32	3653.83 ± 210.84	18.45 ± 2.36	0.9703	8.84	7.11	< 0.0001
Fed-batch	134364.1 ± 7806.24	4454.93 ± 248.35	20.07 ± 2.78	0.9999	77.61	36.10	< 0.0001
Con mode	112251.13 ± 6824.35	3657.56 ± 189.84	19.09 ± 2.87	0.9999	46.73	34.38	< 0.0001

FM	Substrate consumption kinetic parameters by modified Gompertz model								Performance statistics of model
FM	S_max	rs_max	t_L	Yp/s	Yx/s	Yp/x	q_p	q_s	Adj. r²	RMSE	MAE	p-value
Batch	543.46 ± 32.67	20.33 ± 3.53	23.07 ± 4.25	173.05 ± 28.00	1.612 ± 0.06	107.34 ± 31.32	1.118 ± 0.00	0.007 ± 0.01	0.999	3.21	2.46	< 0.0001
Fed-batch	2742.08 ± 198.64	77.56 ± 9.27	20.62 ± 4.03	48.14 ± 11.28	0.752 ± 0.02	65.17 ± 12.55	0.679 ± 0.06	0.018 ± 0.00	0.980	149.85	115.95	< 0.0001
Con mode	2286.62 ± 202.63	135.94 ± 38.74	20.02 ± 2.75	49.09 ± 9.04	0.756 ± 0.03	64.94 ± 12.27	0.676 ± 0.04	0.014 ± 0.00	0.9974	47.86	31.75	< 0.0001

The kinetic parameter values are means of triplicate determinations ± standard error
FM Fermentation mode, Con mode Continuous mode, LGM Logistic model, MGM Modified Gompertz model, P_max maximum l-ASNase concentration (mg), r_pmax maximum volumetric rate of l-ASNase formation (mgL⁻¹ h⁻¹), X₀ initial biomass concentration (gL⁻¹), X_max maximum biomass concentration (gL⁻¹), µ_max maximum specific growth rate (h⁻¹), t_Lag Lag time (h), T_d Doubling time (h), S_max Maximum predicted substrate consumption (gL⁻¹), r_smax maximum volumetric rate of substrate consumption (gL⁻¹ h⁻¹), Adj. r² adjusted coefficient of determination, RMSE root mean squared error, MAE mean absolute error, Yp/s l-ASNase yield relative to amount of substrate consumed, Yx/s Biomass yield relative to amount of substrate consumed, Yp/x Specific l-ASNase activity, qp specific rate of l-ASNase activity, qs Specific rate of substrate consumption, Qp Volumetric productivity of l-ASNase, U(L⁻¹.h⁻¹), Q_x Volumetric biomass productivity, g DCW(L⁻¹.h.⁻¹)