A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli

AMB Express

Table 1 Experiment design by RSM

Response Surface Methodology (RSM) with 3 Factor
Run	Factor 1: OD	Factor 2: Host Cell	Factor 3: IPTG
1	0.6	BL 21 (1)	0.2
2	0.6	BL 21 (1)	0.8
3	1.2	BL 21 (1)	0.5
4	1.8	BL 21 (1)	0.2
5	1.8	BL 21 (1)	0.8
6	0.6	SHuffle (2)	0.5
7	1.2	SHuffle (2)	0.2
8	1.2	SHuffle (2)	0.8
9	1.2	SHuffle (2)	0.5
10	1.2	SHuffle (2)	0.5
11	1.2	SHuffle (2)	0.5
12	1.8	SHuffle (2)	0.5
13	0.6	Nico (3)	0.2
14	0.6	Nico (3)	0.8
15	1.2	Nico (3)	0.5
16	1.8	Nico (3)	0.8
17	1.8	Nico (3)	0.2