Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption

AMB Express

Table 6 The identification results of PCR, microbial culture and ITS sequencing for the 30 Candida clinical isolates

Microbial culture identified isolates (n)	ITS sequencing		PCR
Microbial culture identified isolates (n)	Yes	No	Yes	No
C. albicans(5)	5	0	5	0
C. tropicalis(6)	6	0	6	0
C. glabrata(6)	6	0	6	0
C. parapsilosis(7)	6	1^a	6	1^a
C. krusei(6)	6	0	6	0

^aOne isolate identified as C. parapsilosis by microbial culture was identified as C. krusei both by ITS sequencing and PCR