Table 5 Comparison of linear regression parameters for PCR Ct values obtained from different DNA preparation methods
From: Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption
Species | Slopes (95% CI)a | Significanceb (P value) | y-Intercepts (95% CI)a | Significanceb (P value) | ||
|---|---|---|---|---|---|---|
Conventional | Rapid | Conventional | Rapid | |||
Candida albicans | − 3.125 (− 3.601 to − 2.648) | − 3.287 (− 3.414 to -3.16) | 0.3875 | 37.59 (36.15 to 39.04) | 32.65 (32.26 to 33.03) | < 0.0001* |
Candida tropicalis | − 2.604 (− 3.076 to − 2.132) | − 2.806 (− 3.362 to − 2.25) | 0.4641 | 36.01 (34.58 to 37.44) | 30.43 (28.75 to 32.11) | < 0.0001* |
Candida glabrata | − 2.860 (− 3.519 to − 2.202) | − 3.119 (− 3.38 to − 2.859) | 0.3391 | 37.05 (35.06 to 39.04) | 34.35 (33.56 to 35.14) | < 0.0001* |
Candida parapsilosis | − 2.968 (− 3.44 to − 2.497) | − 2.973 (− 3.268 to − 2.677) | 0.9835 | 37.89 (36.46 to 39.32) | 32.37 (31.48 to 33.27) | < 0.0001* |
Candida krusei | − 3.643 (− 4.033 to − 3.252) | − 3.355 (− 3.518 to − 3.192) | 0.0961 | 38.35 (37.17 to 39.53) | 32.39 (31.9 to 32.88) | < 0.0001* |