Table 4 Species tested by the duplex PCR assay
From: Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption
Species | Sample | Identification methoda | Duplex PCR result |
|---|---|---|---|
Candida albicans SC5314 (ATCCMYA-2876) | Standard strain | – | Candida albicans |
Candida tropicalis ATCC1369 | Standard strain | – | Candida tropicalis |
Candida glabrata ATCC15126 | Standard strain | – | Candida glabrata |
Candida parapsilosis ATCC22019 | Standard strain | – | Candida parapsilosis |
Candida krusei ATCC6258 | Standard strain | – | Candida krusei |
Saccharomyces cerevisiaeATCC9763 | Standard strain | – | Negative |
Escherichia coli(n = 3) | Clinical isolates | Biochemical | Negative |
Klebsiella pneumoniae(n = 3) | Clinical isolates | Biochemical | Negative |
Pseudomonas aeruginosa(n = 3) | Clinical isolates | Biochemical | Negative |
Acinetobacter baumannii(n = 3) | Clinical isolates | Biochemical | Negative |
Enterococcus faecium(n = 3) | Clinical isolates | Biochemical | Negative |
Enterococcus faecalis(n = 3) | Clinical isolates | Biochemical | Negative |
Streptococcus pneumoniae(n = 3) | Clinical isolates | Biochemical | Negative |
Staphylococcus aureus(n = 3) | Clinical isolates | Biochemical | Negative |
Staphylococcus epidermis(n = 3) | Clinical isolates | Biochemical | Negative |
Group B streptococci(n = 3) | Clinical isolates | Biochemical | Negative |
Hepatitis B virus(n = 3) | Positive serum | PCR | Negative |
Hepatitis C virus(n = 3) | Positive serum | PCR | Negative |
Epstein-Barr virus(n = 3) | Positive plasma | PCR | Negative |