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Table 3 Impact of the lysis solution components on PCR amplification

From: Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption

Test NOa

Lysate components

Amount(/50 µL)b

Templates

Ct valuesc

P

–

–

C. albicans DNA

17.36

N

–

–

–

–

1

snailase

16 µg

C. albicans DNA

17.37

2

lyticase

2.5 U

C. albicans DNA

17.36

3

β-mercaptoethanol

0.05 µL

C. albicans DNA

17.28

4

sorbitol

12.5 mM

C. albicans DNA

–

5

EDTA

0.625 mM

C. albicans DNA

–

  1. aP positive control, N negative control
  2. bThe amounts were calculated according to the optimized lysis solution components
  3. cThe Ct (crossing threshold) values describe the point where the amplification curve exceeds the noise band