From: Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption
Test NOa | Lysate components | Amount(/50 µL)b | Templates | Ct valuesc |
---|---|---|---|---|
P | – | – | C. albicans DNA | 17.36 |
N | – | – | – | – |
1 | snailase | 16 µg | C. albicans DNA | 17.37 |
2 | lyticase | 2.5Â U | C. albicans DNA | 17.36 |
3 | β-mercaptoethanol | 0.05 µL | C. albicans DNA | 17.28 |
4 | sorbitol | 12.5 mM | C. albicans DNA | – |
5 | EDTA | 0.625 mM | C. albicans DNA | – |