Table 3 The path of steepest ascent/descent experiments
From: Isolation and production optimization of a novel milk-clotting enzyme Bacillus velezensis DB219
Run | Wheat bran concentration (g/L) | Corn steep liquor concentration (g/L) | Volume (mL) | MCA (SU/mL) |
|---|---|---|---|---|
1 | 50.0 | 2.00 | 50.0 | 2342.86 |
2 | 52.5 | 2.25 | 47.5 | 2433.88 |
3 | 55.0 | 2.50 | 45.0 | 2400.00 |
4 | 57.5 | 2.75 | 42.5 | 2823.53 |
5 | 60.0 | 3.00 | 40.0 | 2954.55 |
6 | 62.5 | 3.25 | 37.5 | 2745.10 |
7 | 65.0 | 3.50 | 35.0 | 2668.73 |
8 | 67.5 | 3.75 | 32.5 | 2560.46 |
9 | 70.0 | 4.00 | 30.0 | 2370.73 |