Fig. 6

The efficiency of transfructosylation by crude inulosucrase. The graph illustrates the transfer of the fructose units contained in the initially applied sucrose into the products in the 10 L scale production process. The upscaled bioconversion reaction from sucrose to I-FOS was started by adding 6000 U L⁻¹ crude inulosucrase to prewarmed solutions of 800 g L⁻¹ household sugar (sucrose), 25 mM sodium acetate buffer (pH 5.5), and 1 mM CaCl₂. The reactions were performed in four replicates at 40 °C for 20 h at a 10 L scale. Sugar and I-FOS concentrations were determined via isocratic HPLC using 58% [v/v] acetonitrile at a flow rate of 0.7 mL min⁻¹