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Fig. 5 | AMB Express

Fig. 5

From: High-yield production and purification of prebiotic inulin-type fructooligosaccharides

Fig. 5

I-FOS production from 570 g L−1 sucrose using crude InuGB-V3. a conversion of sucrose into the products I-FOS, glucose, and fructose. b Progression of the synthesis of single I-FOS of GF2, GF3, GF4, GF5, GF6, and  ≥ GF7. The bioconversion was started by adding 4000 U L−1 crude inulosucrase to a prewarmed solution containing 570 g L−1 sucrose, 25 mM sodium acetate buffer (pH 5.5), and 1 mM CaCl2. The reactions were performed in three biological replicates at 40 °C at a 1 mL scale. Sugar and I-FOS concentrations were determined via isocratic HPLC using 58% [v/v] acetonitrile at a flow rate of 0.7 mL min−1

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