Fig. 1

SDS-PAGE of cell extracts of an InuGB-V3 producing culture and of the purified enzyme. Protein standard: precision Plus Protein Unstained Standard (Bio-Rad), crude InuGB-V3: crude cell extract prepared from an induced culture of E. coli BL21 pASK3_InuGB-V3, control: crude cell extract prepared from a non-induced culture of E. coli BL21 pASK3_InuGB-V3. Cell extract volumes corresponding to 10 µg of total protein were loaded on a Mini-PROTEAN TGX Stain-Free precast gel 12% (Bio-Rad) while 1 µg of the purified enzyme was used for analysis. The proteins were visualized by fluorescent detection with a stain-free enabled imager