Fig. 1From: Homemade plasmid Miniprep solutions for affordable research in low-fund laboratoriesExpression and evaluation of prepared recombinant RNase A. A 15% SDS-PAGE of cell lysates for detection of recombinant RNase A protein expression in BL21 (DE3) cells after IPTG induction. Lane (1) non-induced bacteria lyasate. Lanes (2 and 3): Bacteria lysates after induction for 24 and 48 h. Arrows indicate RNase band. B Evaluation of the activity of the prepared crude recombinant RNase A using Total RNA from HepG2 cells. Lane (1): Total RNA without the RNase A. Lane (2) Total RNA after incubation with the prepared recombinant RNase A. Lane 3 after addition of the RNase inclusion bodiesBack to article page