Table 1 The differential expressed proteins in L. tropica metacyclic and amastigote-like stages

Up-regulated proteins list during metacyclic conversion into amastigote-like form of L. tropica

 12.91

E9ACW0

Putative heat shock protein DNAJ

LMJF_27_2400

DIVHELPVPLEAFYCGK

 7.53

E9ADS8

Putative lipophosphoglycan biosynthetic protein

LMJF_29_0760 (LPG3)

MLDILVNSLYTNR

 2.05

E9AF45

Kinetoplastid membrane protein 11

LMJF_35_2210 (KMPII-1)

FAELLEQQK, LDRLDEEFNRK, EHSEHFK

 3.83

Q4Q1M0

Chaperonin HSP60, mitochondrial

LMJF_36_2030

IQSIHSLLPALNHVVR, TGVTIVR,KIQSIHSLLPALNHVVR, AVAAVATTLGPK

 7.28

Q4Q1R4

Putative universal minicircle sequence binding protein

LMJF_36_1610 (UMSBP1)

CGEAGHMSR

 41.32

Q4Q1Y2

Putative 40S ribosomal protein S18

LMJF_36_0940

SLTLIPDHFQHIVR,FKIPDWFLNR,TEHLSSSMVDTRAGTLTAEELEKIAEIIADPAK, HAYGLR

 13.02

Q4Q3V3

Succinyl-CoA:3-ketoacid-coenzyme A transferase

LMJF_33_2340

SGNLVFR, QTGGQIIR, GPGGAMDLVASGSR

 21.18

Q4Q5P6

Putative 26S proteasome regulatory subunit

LMJF_32_0390

VAGLLLGR, HTNDEAIATFLAAIAR

 22.5

Q4Q822

Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex

LMJF_28_2420

GLVVPVIR, LGLMSPFVK, NLIEDPAR

 6.68

Q4Q931

Putative 40S ribosomal protein S33

LMJF_28_2420 (S33-1)

ENDMLSLMETER, GNVTQVR, LMAEAGSPDYNR

 78.77

Q4Q9X6

ATP synthase subunit beta

LMJF_25_1170

IFNVLGDAIDQR, VAQSALTMAEYFR, GHGGFSVFAGVGER,FTQANSEVSALLGR, TVIIMELINNVAK

 3.0

Q4QAB9

Uncharacterized protein

LMJF_24_2110

ALENPVNLDK, MEFVIDR, NEAAFQDVGIEYYR

 20.39

Q4QD34

Phosphoglycerate kinase

LMJF_20_0100 (PGKC)

SALPTIQK, EGGSCILMSHLGRPK, VLGAGYAGYLMEK

 2.73

Q4QDF0

Glycosomal malate dehydrogenase

LMJF_19_0710 (Gmdh)

RDPALAELAK, GSATLSMAEAGAR,VQVAGTEVVK, DPALAELAK, LLGVSLLDGLR

 5.04

Q4QGX4

Putative pretranslocation protein,alpha subunit

LMJF_11_1050

QANWLMSLKPMLAVLPEIEKPR

 6.15

Q4QJF1

ATPase alpha subunit

LMJF_05_0500

VDAGAPNIVSR, SPVNYNLLTGFK, FVALFNQK, VVNPLGHEVPVGL, AVDTMIPIGR

 2.0

Q9U0V9

Possible 3-ketoacyl-CoA thiolase

LMJF_23_0690 (L7836.03)

LDDFTFPCLFAK, KHPDFGK

 14.28

E9ACG7

Putative delta-1-pyrroline-5-carboxylate dehydrogenase

LMJF_03_0200

YGLTGAVFSR, GAFEFQGQK, CTGAVVGQQPFGGSR, GYFVEPTIIETK

 9.32

E9AFE7

Putative cystathione gamma lyase

LMJF_35_3230

NNLHGGMLWFEVK, VGITDGFVR, NNLHGGMLWFEVK, NNLHGGMLWFEVK

Down-regulated proteins list during metacyclic conversion into amastigote-like form of L. tropica

 8.28

O62591

Probable eukaryotic initiation factor 4A

LMJF_01_0770

HNLIQGLVLSPTR, VLVTTDLVAR,HNLIQGLVLSPTR, ESLTLEGIK

 28.72

Q4FX73

40S ribosomal protein S3a

LMJF_35_0400

NVLSDALVR, FTVQEVQGR, EWYDVVAPANFEK

 4.81

Q4QEB3

GMP reductase

LMJF_17_0725 (GMPR)

IGVGPGSICITR, LIVGAAIGVK, GPLAPILK

 2.47

Q4QG98

60S ribosomal protein L18

LMJF_13_0560 (RPL18-A)

GVDLTGISK, AAPIAVVVGDVLDDVR

 2.84

E9AD27

Putative calpain-like cysteine peptidase

LMJF_27_0500

SIFLPLNTFLK, AELQRAVLKAQNAK, NATAIQDLEEALNDR

 26.07

E9AD53

Putative small GTP-binding protein Rab1

LMJF_27_0760

LLLIGDSGVGK, DFADSLGIPFLETSAK

 3.47

E9ADF9

Putative glycosomal phosphoenolpyruvate carboxykinase

LMJF_27_1810

VAYPLEHIPGALTHAVAGHPNNVIFLTNDAFGVMPPVAR, NLTAPELVQWALK, GALCVL SYAK, KGDVTVFFGLSGTGK, GVFNIEGGCYAK

 17.39

E9ADX3

Tryparedoxin

LMJF_29_1150 (TXN2)

MPWLALPFEDRK

 3.17

E9AE57

Putative fumarate hydratase

LMJF_29_1960

HGGFYLGSIGGPAAILAK, YFAHQAR, YVEEVEVFGR

 2.12

E9AEB3

ATP-dependent 6-phosphofructokinase

pfk

TAIELSR, TIDNDLAFSHR, FGGTILGSSR,HLHFNPSETSIGIVTCGGICPGLNDVIR, EMVDTLVR

 67.7

E9AEL4

Putative ATP-dependent DEAD-box RNA helicase

LMJF_35_0370

TASFVIPVLEK, VHILVATPGR, GFEKPSPVQEEAIPVALQGK, HIPGLEVMVTTGGTTLR, ELALQTAQVTK, NVNFEEYALR

 4.36

E9AEU1

Putative NADH-dependent fumarate reductase

LMJF_35_1180

LGGNSLLECVVFGK, AATILQK, ATSGINAWGTR, LALIGGGTGVAPMLQIVR, LIGCPEANVMATLK

 6.1

E9AF23

40S ribosomal protein S6

LMJF_35_2010

LFNLSR, GAIGFNTFR, RGAIGFNTFR,RVQLQDYR, VGDQPIEGVTDTTAPR

 2.83

E9AFK3

Putative 60S ribosomal protein L23

LMJF_35_3790

VLNAVIIR, ISTHAPAIV, NLYVISVK

 36.1

Q4Q090

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

PGAM

VALQGASLVDDALK, MFVTMDR, SAEITEAAIEALK, VALQGASLVDDALK

 2.64

Q4Q124

Adenosylhomocysteinase

LMJF_36_3910

AGVFFLPK, VAALHLAHVGAK, DISLAEWGR, EHVEIKPQVDR, VKDISLAEWGR, FDNLYGCR

 7.9

Q4Q1D2

40S ribosomal protein S24

S24E-2

TTGFGLIYDDLASLK

 2.8

Q4Q1F5

Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex

LTITPIPMPALSPTMEK, WFQHFHDAMENPLSLLL

 2.49

Q4Q1X7

Putative 40S ribosomal protein S10

LMJF_36_0980

FFFTEGVIACK

 7.71

Q4Q230

Uncharacterized protein

LMJF_36_0480

KSPIMSK, LMDQSLPVYDDVVTGVGR

 2.59

Q4Q2H7

Putative vacuolar ATP synthase catalytic subunit A

LMJF_34_3670

ITWNYIR, NIVTFYEEAQR, TCLVANTSNMPVAAR, EEELQEIVQLVGK

 7.61

Q4Q3U8

Putative heat shock protein

LMJF_33_2390

YNLHFNPQHPLIR, GLLPDWLR, EELTANLGTIAGSGSK

 2.74

Q4Q4U1

Dihydrolipoyl dehydrogenase

GCVL-2

ALTGGVEYLFK, AAQLGLK, AVGTEDGFVK

 3.42

Q4Q5P0

40S ribosomal protein S2

LMJF_32_0450

GTGIVAAPVPK, THGNLIMATFYALR

 8.42

Q4Q6E1

Putative vacuolar-type proton translocating pyrophosphatase 1

LMJF_31_1220

QFQDPEVAEGR

 3.81

Q4Q9H4

Putative 60S ribosomal protein L7

KILQLLR, AVEPYIAYGYPSLATVR

 35.55

Q4Q9M4

Succinate–CoA ligase [ADP-forming] subunit alpha, mitochondrial

LMJF_25_2130

VIVQGMTGK, VVGGVSPK, VIVQGMTGK, AGTFHTK

 3.93

Q4Q9R2

Polyprenol reductase

EnCR

DLGPQIGYR, ELESMFVHK, FSHPTMPMR

 2.22

Q4Q9V1

GTP-binding nuclear protein

LMJF_25_1420

LILVGDGGTGK, SNYNFEKPFVWLAK, VCDNIPIVLVGNK

 5.74

Q4Q9Y0

Putative cytochrome c oxidase VII

LMJF_25_1130

IPNPFAYSFK, VWAPATTLAEYR

 5.22

Q4QAG8

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

LMJF_24_1630

AITMEILAGR, LGANSLLDIVVFGK, GEGGYLVNSEGER, SPVWNSNLIEALELR

 18.71

Q4QAX6

Putative 60S ribosomal protein L17

LMJF_24_0040

HVQVDQAAR, SVVAMMSLLK

 2.0

Q4QEI9

Elongation factor 1-alpha

LMJF_17_0080

IGGIGTVPVGR, GITIDIALWK, FESPKSVFTIIDAPGHR, SVFTIIDAPGHR, EHALLAFTLGVK, STATGHLIYK

 7.48

Q4QEM2

Paraflagellar rod protein 2C

LMJF_16_1425

AQLLEHLVELVADKFR, TLGQLVYK

 14.07

Q4QEX4

Putative 60S ribosomal protein L21

LMJF_16_0460

GVGVIINKPVR, TGIVWNVTPR, VGDYVDVVADSAVR

 33.52

Q4QF62

60S acidic ribosomal protein P2

LMJF_15_1203

AVHIDVDQATLAFVMESVTGR, ASPSQADVEAICK

 23.38

Q4QF80

Tryparedoxin peroxidase

TRYP1

GLFIIDPHGMLR

 6.15

Q4QFF2

Putative ribonucleoprotein p18, mitochondrial

LMJF_15_0280

FCAMMDLMEEMQHR, FCAMMDLMEEMQHR, NCPPDLETYNATLQK

 19.25

Q4QFG2

Putative 60S ribosomal protein L13a

LMJF_15_0200

APSDVFVR, HRPEIIVIDLK, HRPEIIVIDLKDHVLGR, CEQLNIAGTEIR

 2.34

Q4QFL8

Enolase

ENOL

HIDEPLPILMEAIEK, LPVPCFNVINGGK

 181.11

Q4QFP8

Putative small myristoylated protein-3

SMP-3

ISFEANPIAK, DNGNGLLFR

 5.92

Q4QG31

40S ribosomal protein S4

RS4

LRECLPLLVIIR, AVIVTGGANR, ECLPLLVIIR,DLNNLQVTVPK, MNVIQER,DASGAEFATR

 3.49

Q4QGA9

Uncharacterized protein

LMJF_13_0450

SPEFDAIYEQQQK

 2.77

Q4QGC5

Tubulin alpha chain

LMJF_13_0280

LIGQVVSSLTASLR, IHFVLTSYAPVVSAEK, EIVDLALDR, QLFNPEQLVSGK, AVCMIANSTAIAEVFAR

 2.59

Q4QGN9

Glucose-6-phosphate isomerase

PGI

AVLHVALR, HFVALSTNTEK, PSNSILVNALTPR, QVNLEETIFIIASK

 22.93

Q4QIP1

Putative 60S ribosomal protein L7a

LMJF_07_0500

APLAVVTGLQEVTR, WPTFVTMQR,TATCVALTDVNAEDEATLK