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AMB Express

Table 2 Mean fluorescence intensity change vs. intensity It0 (%) at different potentials (V) of dyes ConA488 and WGA555 under different conditions

From: Suitability of lectin binding studies for the characterization of redox-active microbial environmental biofilms

 

ConA488

WGA555

Potential

(1)

(2)

(3)

(1)

(2)

(3)

Reduction sweep

 0.2

0.8

1.5

0

− 0.1

0.9

0.0

 0

0.8

1.5

0.7

0.6

2.8

2.2

 − 0.2

1.6

2.4

1.8

2.0

6.5

5.7

 − 0.5

2.7

4.6

3.9

2.2

9.5

11.5

Oxidizing sweep

 0.7

2.3

3.7

3.2

4.3

4.1

6.0

 0.9

2.0

1.2

0.0

3.7

0.8

0.3

 1.1

− 2.4

− 3.9

− 6.2

− 1.9

− 4.3

− 6.5

  1. Stained biofilm (1), fluorescence probe in filtered biofilm water (2), and fluorescence probe in filtered biofilm water, buffered with 0.1 M MES (3). Potentials are listed in sequence of appearance during cycling
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