Fig. 5
From: Housekeeping gene gyrA, a potential molecular marker for Bacillus ecology study
The results of amplicon sequencing for the mock community including eight Bacillus strains. A PCR amplification of eight Bacillus strains by gyrA gene primer pairs. The orange square indicates positive and white square unsuccessful amplification. The amplicon sequencing results of 16S rRNA gene B and gyrA gene using primer pairs gyrA3 C, gyrA4 D, and gyrA5 E for the mock community. The relative abundance is represented by reads numbers for each unit as indicated on the X axis. The phylogenetic tree was contracted based on the complete gyrA genes (2469 bp) (Additional file 2: Dataset S2) by using the Neighbor-Joining method in MEGA 5.05 software. The reliability of clades was tested by the 1000 bootstrap replications. The box plots were drawn by the ggplot2 R package (v.3.2.1)