Fig. 3
From: Housekeeping gene gyrA, a potential molecular marker for Bacillus ecology study
The amplification ability of the primer pair gyrA3 of Bacillus species and related species performed by computer simulation and in situ amplification. The Bacillus species indicated by the orange color (48 species) represent the species in the database that were matched by gyrA3 only in virtual PCR. The Bacillus species in blue color represented strains that can be amplified by gyrA3 only by colony PCR. These strains included 21 Bacillus species and 16 Bacillus’s related genera species. The Bacillus species in green color (7 species) represent strains that can be amplified by two methods: virtual PCR and colony PCR. The species in black color (2 species) represent strains that cannot be amplified in virtual PCR nor by colony PCR. The phylogenetic tree was constructed based on 16S rRNA (1302 bp)