Fig. 1

Sliding window analysis of nucleotide variability [Pi (π)] along the sequence of the gyrA gene (A) and 16S rRNA (B) in Bacillus. 361 and 256 of gyrA and 16S sequences from 20 species and three species (B. amyloliquefaciens (n = 88), B. licheniformis (n = 71) and B. pumilus (n = 97)) were aligned with the Kalign method and nucleotide diversity was determined in DnaSP (v.6) using a window size of 100-bp, a step size of 10-bp, and points based on the mid-point of each window (i.e., the first point is at position 200). The blue lines display the Bacillus inter-species nucleotide diversity of the two genes and the orange, red and yellow lines display the nucleotide diversity of two genes in B. amyloliquefaciens, B. licheniformis and B. pumilus, respectively. The sequence length of the gyrA gene for analysis was 2450 bp, and that of 16S rRNA was 1540 bp