Recombinant acetylxylan esterase of Halalkalibacterium halodurans NAH-Egypt: molecular and biochemical study

Embaby, Amira M.; Mahmoud, Hoda E.

doi:10.1186/s13568-022-01476-w

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Table 2 Comparison between some reported AXE(s) and AXE-HAS10 regarding biochemical properties

From: Recombinant acetylxylan esterase of Halalkalibacterium halodurans NAH-Egypt: molecular and biochemical study

Microbial source	Enzyme designation	Expression host	a.a^a	Optimal pH	Optimal Temp^b. (^oC)	M.W^c (kDa)	CE Fam.^d	PDB accession number	Seq. Iden. (%) with AXE-HAS10	Activity at lowest temp (^oC)	Thermost-ability	Kinetic Parameters on p-NP-C2	Reference
Ochrovirga pacifca	rAXE	E. coli	287	8.3	50	31.75	N. A	N. A	16.2	40% activity at 25 ^oC	100% activity at 45 ^oC for 2 h	N. A	Hettiarachchi et al. 2019
Lactobacillus antri	LaAXE	E. coli	323	7.0	50	37.00	CE7	N. A	37.8	65% activity at 30 ^oC	N. A	N. A	Kim et al. 2020
Bacillus halodurans C-125	BhAXE	E. coli	319	8.0	50	36.00	CE7	N. A	100	45% activity at 30 ^oC	N. A	N. A	Kim et al. 2020
Thermoanerobacterium sp.	rAXE1	E. coli	963	7.0	75–80	32.00	CE7	3FCY	63.3	N. A	60% activity at 75 ^oC for 3 h	N. A	Shao and Wiegel 1995 & Lorenz and Wiegel 1997
Clostridium saccharobutylicum	EST1051	E. coli	307	7.0	40	35.00	CE7	N. A	40.0	30% activity at 4 ^oC	60% activity at 60 ^oC for 24 h	Km (0.923 mM)	Xu et al. 2021
Termite hindgut metagenome	AXEA	E. coli	325	7.5	40	40.00	CE7	N. A	54.9	30% activity at 30 ^oC	50% activity at 60 ^oC for < 1 h	Km (0.1 mM)	Mokoena et al. 2015
Termite hindgut metagenome	AXEB	E. coli	322	7.5	40	40.00	CE7	N. A	32.6	30% activity at 30 ^oC	50% activity at 60 ^oC for < 1 h	Km (0.23 mM)	Mokoena et al. 2015
Halalkalibacterium halodurans NAH-Egypt	AXE-HAS10	E. coli	319	8.5	40	41.39	CE7	N. A	100	22% activity at 5 ^oC	100 & 82% activity at 35 ^oC for 1 and 2 h, respectively	Km (0.096 mM)	This study
Bacillus pumilus	AXE	E. coli	320	8.0	55	40.0	CE7	2xlb, 3FYT, and 3FVT	38.0	N. A	50% activity at 60 ^oC for 1 h	Km (1.54 mM)	Degrassi et al. 2000 Degrassi et al. 1998
B. subtilis	AXE	E. coli	318	8.0	50	35.6	CE7	1L7A, 1ODS, and 1ODT	36.8	65% activity at 30 ^oC	100% activity at 40 ^oC for 2 h	N. A	Tian et al. 2014
Thermotoga maritima	AxeA	E. coli	336	6.5	90	37.0	CE7	1VLQ, 3M81, 3M82, and 5GMA	31.6	20% activity at 30 ^oC	50% activity at 98 ^oC for 13 h & 90 ^oC for 67 h	Km (0.12 mM)	Drzewiecki et al. 2010
Penicillium chrysogenum P33	PcAxe	Pichia pastoris	267	7.0	40	31.6	CE1	N.A	15.1	58% activity at 20 ^oC	< 20% activity at 80 ^oC for 1 h	Km (0.465 mM)	Yang et al. 2017
Volvariella volvacea	VvaxeII	E. coli	253	7.0	60	27.8	CE4	N.A	3.1	N.A	N. A	N. A	Liu and Ding 2009
Aspergillus oryzae	rAoAXE	Pichia pastoris	276	6.0	45	31.0	CE1	N.A	20.4	75% activity at 30 ^oC	25% activity at 50 ^oC after 1 h	N. A	Koseki et al. 2006
Aspergillus niger	AXE	Aspergillus niger	277	5.5-6.0	50	30.4	CE1	N. A	24.3	N. A	95% activity at 50 ^oC for 2 h	N.A	Kormelink et al., 1993
Streptomyces lividans IFA43	AxeA	Streptomyces lividans IFA43	309	7.5	70	34.0	CE4	3COO	35.7	10% activity at 30 ^oC	50% activity at 72 ^oC for 15 min	N.A	Dupont et al. 1996

a: amino acids residues, b: temperature, c: experimental molecular weight, d: carbohydrate esterase family
N.A: not available

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