Table 1 Purification table and kinetic parameters for hydrolysis of p-NP-C2 by recombinant AXE-HAS10
Status | Vol. (mL) | Total units | Total mg protein | Specific activity (U/mg) | Fold | Yield (%) | Km (mM) | kcat (s− 1) | kcat/Km (s− 1.mM−1) |
|---|---|---|---|---|---|---|---|---|---|
Crude soluble cell lysate | 1.0 | 43.08 | 13.30 | 3.23 | 1.00 | 100.00 | ND | ND | ND |
Ni2+ affinity chromatography | 9.0 | 30.95 | 0.837 | 36.99 | 11.450 | 71.84 | 0.096 | 63.06 | 6.571 × 102 |