Fig. 4From: Efficient depolymerization of polyethylene terephthalate (PET) and polyethylene furanoate by engineered PET hydrolase Cut190Effect of surfactant concentrations on amorphous PET film and amorphous PET powder. A Two pieces (6 mm ∅; 116 μmoles/ml as monomer unit) of amorphous PET film (Nonacryl V): B Homogenized PET bottle flakes (28.5 μmoles/ml as monomer unit): C Powder 2 (105 μmoles/ml as monomer unit). The reactions were performed at 63 °C for 3 days in a 1-ml reaction mixture, using Cut190*SS under the standard reaction conditions except that buffer concentration in A (50–100 ppm) (160–310 μM) and C (0–100 ppm) (0–310 μM) was raised to 150 mM. The reactions were performed in duplicate. Variations in values are indicated by open circles for total products and open squares for degradation (%)Back to article page