Fig. 4

(A) Effect of pH on enzyme activity. (B) Effect of pH on enzyme stability. The activities were determined by assays with CMC as substrate following incubation of the enzyme at pH 4 (■), pH5(●), pH 6 (▶) and pH 7 (◀) for the indicated times. (C) Effect of temperature on enzyme activity. (D) Effect of temperature on enzyme stability. The activities were determined by assays with CMC as substrate following incubation of the enzyme at 85 °C (▲), 90 °C (●), and 95 °C (■) for the indicated times. (E) Differential Scanning Calorimetry (DSC) analysis of BcelFp. The enzyme was concentrated to 1.5 mg/ml in 50 mM PBS buffer (pH 6.0). The equilibrated enzyme was scanned from 35 to 120 ^oC at a rate of 2.0 K/min. The enzyme scan was corrected using a buffer–buffer baseline