Modification and application of highly active alkaline pectin lyase

AMB Express

Table 1 Comparison of properties of other reported pectin lyases

Pectin lyase	pH	Temperature/(°C)	Enzyme activity^a/(U/mL)	Specific activity/(U/mg)	Culture medium	Expression host	Source of bacteria	References
BspPel	10	80	310	127.9	YPD medium	Pichia pastoris	Bacillus sp. RN1	Zheng et al. (2020)
Pel4J4	8.5	55	204.4	1059.4	LB medium	E. coli BL21	D.dadantii DCE-01	Cheng et al. (2019)
BacPelA	10.5	70	490.2	675.5	TB medium	E. coli BL21	B.clausii S10	Zhou et al. (2017a)
PpPel9a	10	40		298.5	LB medium	E. coli BL21	Paenibacillus Polymyxa KF-1	Yuan et al. (2019)
BliPelA	11	70	85.2	320	Modified TB medium	E. coli BL21	Bacillus licheniformis 91	Zhou et al. (2017c)
recPel S6	10	60		49.6	LB medium	E. coli BL21	Bacillus amyloliquefaciens S6	Bekli et al. (2019)
PGLA-rep1	10.5	70	413.8	604.9	TB medium	E. coli BL21	B.clausii S10	This work
PGLA-rep2	10.5	70	434.4	680.9	TB medium	E. coli BL21	B.clausii S10	This work
PGLA-rep3	11	65	433.0	472.5	TB medium	E. coli BL21	B.clausii S10	This work
PGLA-rep4	11	70	554.0	822.9	TB medium	E. coli BL21	B.clausii S10	This work

^aThe activity was determined by the A235 method, and the average value of three experiments was expressed