Fig. 2
From: DAR-PCR: a new tool for efficient retrieval of unknown flanking genomic DNA
Walking upstream of gadA(U) and hyg (U), as well as downstream of hyg (D). The ISA primers listed under each gene were respectively paired with SSP1 during the primary PCR reactions; the corresponding secondary PCRs were then performed using SSP2 and SSP3 (as described in Table 1). Lane 1: primary PCR; lane 2: secondary PCR; white arrows indicate target bands; and M: DL5000 DNA marker