Fig. 4

Effect of deletions of creA, pkaA, and ganB on growth and the production of β-mannanase. Precultured strains were further grown in MM containing LBG with or without d-glucose (LBG + glc or LBG) for 6 h and 12 h, and dry mycelial weight (a) and total β-mannanase activity in the culture supernatant (b) were measured. White bars, the reference strain; light gray bars, ΔcreA; dark gray bars, ΔpkaA; filled bars, ΔcreAΔpkaA; dashed bars, ΔganB; striped bars, ΔcreAΔganB. Error bars indicate the standard deviations of three independent experiments. Different letters indicate significant differences at P < 0.05 by one-way ANOVA with Tukey’s post hoc test