Table 1 Primer sequence used for the cytokine gene expression
Gene | Type | Sequence | Product Size (bp) | Annea-ling Temp | References |
|---|---|---|---|---|---|
GAPDH | Forward | 5′-CTGCCCAGAACATCATCCCT-3′ | 266 | 61 °C | (Stephens et al., 2011) |
Reverse | 5′-GGTCCTCAGTGTAGCCCAAGA-3′ | ||||
TLR2 | Forward | 5′-TCGTTGTTCCCTGTGTTGCT-3′ | 389 | 65 °C | (Oshikawa and Sugiyama, 2003) |
Reverse | 5′-CCACGCCCACATCATTCTCA-3′ | ||||
iNOS | Forward | 5′-CACCTTGGAGTTCACCCAGT-3′ | 170 | 60 °C | (Chaturvedi et al., 2007) |
Reverse | 5′-ACCACTCGTACTTGGGATGC-3′ | ||||
IL-β1 | Forward | 5′-ATGGCAACTGTTCCTGAACTCAACT-3′ | 563 | 60 °C | |
Reverse | 5′-CAGGACAGGTATAGATTCTTTCCTTT-3′ | ||||
IFN-γ | Forward | 5′-GGTTGGACAAAAAGAATCTG-3′ | 227 | 55 °C | (Munder et al., 2002) |
Reverse | 5′-ACCACAGAGAGCAAGGACTT-3′ | ||||
IL-6 | Forward | 5′-GCCTATTGAAAATTTCCTCTG-3′ | 310 | 59 °C | (Tsoi et al., 2021) |
Reverse | 5′-TAGGTTTGCCGAGTAGATCTC-3′ | ||||
IL-12 | Forward | 5′-CGT GCT CAT GGC TGG TGC AAA G-3′ | 220 | 61 °C | (Sisto et al., 2003) |
Reverse | 5′-CTT CAT CTG CAA GTT CTT GGG C-3′ | ||||
IL-4 | Forward | 5′-TCG GCA TTT TGA ACG AGG TC-3′ | 216 | 57 °C | (Sisto et al., 2003) |
Reverse | 5′-GGTTGGACAAAAAGAATCTG-3′ | ||||
TNF-α | Forward | 5′-GAGCTTTCAACAACTACTCAG-3′ | 276 | 58 °C | (Sisto et al., 2003) |
Reverse | 5′-GGAAGGCCTGAGATCTTATC-3′ | ||||
IL-10 | Forward | 5′-CGGGAAGACAATAACTG-3′ | 186 | 62 °C | (Sisto et al., 2003) |
Reverse | 5′-CATTTCCGATAAGGCTTGG-3′ |