Table 3 ANOVA analysis of SAK expression in dicistronic Hsp27 SILEX system by the second order polynomial model
From: Indirect optimization of staphylokinase expression level in dicistronic auto-inducible system
Source | Sum of squares | df | Mean square | F-value | P-value | Significant |
|---|---|---|---|---|---|---|
Model | 27.30 | 11 | 2.48 | 28.17 | < 0.0001 | * |
A-Temperature | 11.39 | 1 | 11.39 | 129.25 | < 0.0001 | * |
B-Inoculation | 1.42 | 1 | 1.42 | 16.08 | 0.0004 | * |
C-Media | 4.75 | 2 | 2.38 | 26.99 | < 0.0001 | * |
AB | 1.50 | 1 | 1.50 | 17.02 | 0.0003 | * |
AC | 1.31 | 2 | 0.6575 | 7.46 | 0.0026 | * |
BC | 0.5888 | 2 | 0.2944 | 3.34 | 0.0505 | |
A2 | 3.07 | 1 | 3.07 | 34.84 | < 0.0001 | * |
B2 | 3.27 | 1 | 3.27 | 37.07 | < 0.0001 | * |
Residual | 2.38 | 27 | 0.0881 | |||
Lack of Fit | 1.19 | 15 | 0.0797 | 0.8075 | 0.6572 | |
Pure Error | 1.18 | 12 | 0.0986 | |||
Cor Total | 29.67 | 38 |