Indirect optimization of staphylokinase expression level in dicistronic auto-inducible system

AMB Express

Table 1 The designed experiments for indirect optimization of SAK expression as a response

Run	Temperature (°C)	Inoculum load	Culture medium	Experimental eGFP Expression level (µg/ml)
1	23	0.040	TB	4.9
2	23	0.040	LB	3.8
3	37	0.050	LB	1.3
4	39	0.040	2YT	3.5
5	25	0.050	TB	5.2
6	25	0.050	2YT	4.0
7	37	0.030	LB	1.7
8	31	0.040	2YT	2.9
9	25	0.050	LB	3.8
10	39	0.040	LB	2.3
11	31	0.040	TB	2.7
12	37	0.050	2YT	2.3
13	25	0.030	2YT	2.8
14	31	0.040	2YT	3.5
15	31	0.027	2YT	2.3
16	25	0.030	LB	2.9
17	31	0.040	2YT	2.6
18	31	0.040	LB	2
19	31	0.040	TB	3.3
20	31	0.040	TB	3.3
21	39	0.040	TB	2.8
22	25	0.030	TB	3.2
23	37	0.030	2YT	2.6
24	31	0.040	2YT	3.3
25	31	0.040	LB	2.1
26	31	0.040	LB	2.0
27	23	0.040	2YT	4.3
28	31	0.040	LB	2.1
29	31	0.040	TB	3.3
30	31	0.027	LB	1.6
31	31	0.027	TB	1.9
32	31	0.040	LB	2.8
33	37	0.050	TB	2.3
34	31	0.040	TB	3.5
35	37	0.030	TB	1.6
36	31	0.040	2YT	3.2
37	31	0.053	TB	2.6
38	31	0.053	LB	2.1
39	31	0.053	2YT	2.3