Table 1 Primer sequences used for PCR amplification and sequence analysis
From: Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis
Gene | Primer | Sequence | Amplified fragment size bp) | Temp (°C) | References |
|---|---|---|---|---|---|
rpoB | MYCOF | 5'-GGCAAGGTCACCCCGAAGGG-3' | 750 | 68 | Adekambi et al. 2003 |
MYCOR | 5'-AGCGGCTGCTGGGTGATCATC-3ʹ | 68 | |||
argH | ARGHF | 5ʹ-GACGAGGGCGACAGCTTC-3' | 629 | 60 | Macheras et al. 2009 |
ARGHS | 5ʹ-GTGCGCGAGCAGATGATG-3' | 58 | |||
cya | ACF | 5'-GTGAAGCGGGCCAAGAAG-3' | 647 | 58 | Macheras et al. 2009 |
ACFR1 | 5'-AACTGGGAGGCCAGGAGC-3' | 60 |