Table 4 End-products produced by the N. frontalis + M. gottschalkii co-culture YakQH5 and the N. frontalis YakQH5 fungus grown on the 5 substrates

N. frontalis + M. gottschalkii Co-culture YakQH5

CH₄ (mmol/g DM)

Day2

1.5 ^a

1.0 ^b

1.7 ^a

0.9 ^b

1.2 ^b

Day3

2.8 ^a

2.6 ^a

3.0 ^a

2.1 ^b

2.3 ^b

Day4

3.8 ^a

3.9 ^a

3.5 ^a

2.5 ^b

2.9 ^b

Day5

4.6 ^a

4.2 ^a

3.9 ^ab

4.0 ^ab

3.0 ^b

Average

3.2 ^a

2.9 ^a

3.0 ^a

2.4 ^b

2.4 ^b

Acetate (mmol/g DM)

Day2

2.0 ^b

1.9 ^c

2.1 ^b

2.2 ^b

2.9 ^a

Day3

4.0 ^b

4.0 ^ab

4.6 ^a

3.7 ^b

5.0 ^a

Day4

6.2 ^b

6.0 ^b

7.1 ^a

5.9 ^c

6.5 ^b

Day5

8.0 ^b

7.3 ^c

8.6 ^a

7.3 ^c

8.2 ^b

Average

5.1 ^b

4.8 ^c

5.6 ^a

4.8 ^c

5.7 ^a

N. frontalis YakQH5 Fungus

H₂ (mmol/g DM)

Day2

0.5 ^a

0.2 ^b

0.4 ^a

0.2 ^b

0.5 ^a

Day3

1.8 ^b

1.0 ^d

1.5 ^c

2.0 ^a

1.7 ^b

Day4

2.8 ^a

2.1 ^c

1.9 ^d

2.7 ^a

2.5 ^b

Day5

3.9 ^a

2.5 ^c

2.4 ^c

3.0 ^b

2.8 ^b

Average

2.0

1.5

1.6

2.0

1.8

Formate (mmol/g DM)

Day2

0.4 ^b

0.4 ^b

0.6 ^a

0.3 ^c

0.6 ^a

Day3

1.1 ^b

1.1 ^b

1.2 ^a

1.1 ^b

1.3 ^a

Day4

1.7 ^b

1.6 ^b

1.6 ^b

1.6 ^b

1.9 ^a

Day5

1.9 ^c

2.0 ^b

2.1^b

1.9 ^c

2.5 ^a

Average

1.3 ^c

1.3 ^c

1.4 ^b

1.2 ^d

1.6 ^a

Acetate (mmol/g DM)

Day2

1.5 ^a

1.0 ^c

1.5 ^a

1.3 ^b

1.2 ^b

Day3

2.8 ^a

1.9 ^c

2.7 ^a

2.0 ^b

2.1 ^b

Day4

4.0 ^a

3.3 ^b

4.1 ^a

3.1 ^b

3.2 ^b

Day5

5.3 ^a

4.1 ^c

5.5 ^a

4.4 ^b

4.6 ^b

Average

3.4 ^a

2.6 ^b

3.5 ^a

2.7 ^b

2.8 ^b

Lactate (mmol/g DM)

Day2

0.6 ^c

0.8 ^a

0.5 ^d

0.5 ^d

0.7 ^b

Day3

1.3 ^b

1.5 ^a

1.2 ^b

1.2 ^b

1.2 ^b

Day4

1.9 ^b

2.4 ^a

1.8 ^b

1.6 ^c

1.7 ^c

Day5

2.1 ^b

2.4 ^a

1.9 ^c

1.9 ^c

2.5 ^a

Average

1.5 ^b

1.8 ^a

1.4 ^c

1.3 ^c

1.5 ^b

Ethanol (mmol/g DM)

Day2

10.5 ^a

8.0 ^b

6.3 ^c

9.5 ^b

7.0 ^c

Day3

30.3 ^a

23.7 ^b

19.1 ^c

29.7 ^a

25.0 ^b

Day4

Day5

Average

37.1 ^a

45.8 ^a

30.9 ^a

30.3 ^b

35.0 ^c

24.3 ^c

24.7 ^c

27.9 ^d

19.5 ^d

34.6 ^a

39.9 ^b

28.4 ^b

30.1 ^b

33.4 ^c

23.9 ^c