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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Flow cytometry-based viability staining: an at-line tool for bioprocess monitoring of Sulfolobus acidocaldarius

Fig. 3

Comparison of viability measurements of Sulfolobus acidocaldarius in response to the shift in pH value, observed over time [h]. The viability was determined by flow cytometry (VFCM [%]) and by plating assay (Vplating [%]). Both pH shifts from 3.0 to 2.0 and to 1.5, respectively caused a drop in both viability-determining methods as well as in OD600. In this case, Vplating measured at the beginning of the experiment was set to 100%, and for determining Vplating [%], the CFUs of each sample were then divided by this initial value

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