Table 1 Influence of medium composition on G. zeae protoplast formation
From: Ursodeoxycholic acid production by Gibberella zeae mutants
Medium no | Nutrient components, g/L | Protoplast yield/mL | ||||||
|---|---|---|---|---|---|---|---|---|
Sucrose | Glucose | Yeast extract | Soy flour | Peptone | KH2PO4 | MgSO4 | ||
1 | 20 | – | – | – | – | 1 | 0,5 | 0.3 (± 0.02) × 104 |
2 | 20 | – | 5 | – | – | 1 | 0,5 | 0.2 (± 0.01) × 105 |
3 | – | 20 | 5 | – | – | 1 | 0,5 | 0.6 (± 0.03) × 105 |
4 | – | 20 | – | 10 | – | 1 | 0,5 | 0.7 (± 0.04) × 103 |
5 | – | 20 | – | – | 10 | 1 | 0,5 | 0.2 (± 0.01) × 104 |