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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Modulation of osteogenic differentiation by Escherichia coli-derived recombinant bone morphogenetic protein-2

Fig. 1

Effects of E-rhBMP-2 and C-rhBMP-2 on intracellular receptor-regulated Smads in C2C12 cells. A total of 0.2 mg protein was loaded onto each lane and anti-phospho-Smad1/5/9 antibody was applied to detect the phospho-Smad1/5/9. Bands for Smad1 and β-actin are shown as the loading control. Bands were detected using Amersham Imager 600 (GE Healthcare). a E-rhBMP-2, Escherichia coli-derived recombinant human bone morphogenetic protein-2; b C-rhBMP-2, rhBMP-2 derived from CHO cell; c Relative phosphorylation was calculated normalizing the quantification values of phospho-Smad1/5/9 and Smad1 band intensity to the β-actin band intensity

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